Cell Biology Platform

Research director Guido KROEMER
Scientific co-director: Oliver KEPP 

• Contact details

Gustave Roussy, BioCell platform, 114, rue Edouard Vaillant, 94805 Villejuif
Tel. office: +33(0)1 42 11 45 16
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• Team members:

- Scientific director : Oliver KEPP

- Lab manager : Sabrina FORVEILLE

- Data Scientists : Allan SAUVAT; Marion LEDUC

- PostDocs : Lucillia BEZU; Yan WANG; Peng LIU; Qi WU; Liwei ZHAO

- PhD students : Wei XIE; Juliette HUMEAU; Giulia CERRATO; Hui Pan; Shuai Zhang; Ai-Ling Tian

Summary of the research topics :

Built within the Paris Alliance of Cancer Research Institutes (PACRI), the BioCELL platform works in concert with INSERM UMR1138 “APOPTOSIS, CANCER & IMMUNITY” to investigate the molecular and cellular mechanisms of cell stress and cell death. The mode of cellular stress and death that is evoked during cancer treatment has profound therapeutic implications. A systematic search has been launched to identify molecular switches that “decide” which particular cell stress or cell death subroutine is preferentially executed. In addition, cancer cell death modalities and their impact on the immune system are analyzed in a systematic fashion to develop strategies that improve the chances of therapeutic success.
The Cell Biology platform at Gustave Roussy is used as a hub to conduct screening approaches, as it offers fully automated cell biology workflows that allow for the phenotypic screening of large-scale compound and siRNA libraries necessary to conduct systems biology oriented research. The unique design of the platform allows for extremely flexible assay design and multiplexing as it integrates automated cell culture and compound management with three different fully automated high content analysis systems via an industrial robot.

Keywords:

High throughput compound and siRNA screening; imaging; biosensors; cell death

Top 5 recent publications:

• Liu P, Zhao L, Pol J, Levesque S, Petrazzuolo A, Pfirschke C, Engblom C, Rickelt S, Yamazaki T,  et al. (2019) Crizotinib-induced immunogenic cell death in non-small cell lung cancer. Nat Commun. Apr 17;10(1):1883 
• Gomes-da-Silva LC, Zhao L, Bezu L, Zhou H, Sauvat A, Liu P, et al. (2018) Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus. The EMBO Journal, Jul 2;37(13)
• Niso-Santano, M., Malik, S.A., Pietrocola, F., Bravo-San Pedro, J.M., Marino, G., Cianfanelli, V., Ben-Younes, A., Troncoso, R., Markaki, M., Sica, V., et al. (2015). Unsaturated fatty acids induce non-canonical autophagy. The EMBO journal 34, 1025-1041.
• Lissa, D., Senovilla, L., Rello-Varona, S., Vitale, I., Michaud, M., Pietrocola, F., Boileve, A., Obrist, F., Bordenave, C., Garcia, P., et al. (2014). Resveratrol and aspirin eliminate tetraploid cells for anticancer chemoprevention. Proceedings of the National Academy of Sciences of the United States of America 111, 3020-3025.
• Sistigu, A., Yamazaki, T., Vacchelli, E., Chaba, K., Enot, D.P., Adam, J., Vitale, I., Goubar, A., Baracco, E.E., Remedios, C., et al. (2014). Cancer cell-autonomous contribution of type I interferon signaling to the efficacy of chemotherapy. Nature medicine 20, 1301-1309.
• Senovilla, L., Vitale, I., Martins, I., Tailler, M., Pailleret, C., Michaud, M., Galluzzi, L., Adjemian, S., Kepp, O., Niso-Santano, M., et al. (2012). An immunosurveillance mechanism controls cancer cell ploidy. Science 337, 1678-1684.