Cell Biology Platform

Research director Guido KROEMER
Scientific co-director: Oliver KEPP 

• Contact details

Gustave Roussy, Cell Biology platform, 114, rue Edouard Vaillant, 94805 Villejuif

OncoPhenoScreen platform, 15 Rue de l'École de Médecine, 75006 Paris

Tel. office: +33(0)1 42 11 45 16

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• Team members:

- Scientific director : Oliver KEPP

- Technical director : Allan Sauvat

- Lab manager : Sabrina FORVEILLE

- Data Scientists : Marion LEDUC

- PostDocs : Lucillia BEZU; Liwei ZHAO

Summary of the research topics :

Built within the Paris Alliance of Cancer Research Institutes, the Cell Biology and OncoPhenoScreen platforms work in concert with INSERM UMR1138 “APOPTOSIS, CANCER & IMMUNITY” to investigate the molecular and cellular mechanisms of cell stress and cell death. The mode of cellular stress and death that is evoked during cancer treatment has profound therapeutic implications. A systematic search has been launched to identify molecular switches that “decide” which particular cell stress or cell death subroutine is preferentially executed. In addition, cancer cell death modalities and their impact on the immune system are analyzed in a systematic fashion to develop strategies that improve the chances of therapeutic success.
The Cell Biology platform at Gustave Roussy and the OncoPhenoScreen platform are used as hubs to conduct screening approaches, as they offer fully automated cell biology workflows that allow for 3D-phenotypic screening of large-scale compound and siRNA libraries necessary to conduct systems biology oriented research. The design of the platforms allows for extremely flexible assay design and multiplexing as they integrate automated cell culture and compound management with multiple different fully automated high content analysis systems via industrial robots.

Keywords:

High throughput compound and siRNA screening; imaging; biosensors; cell death

Top 5 recent publications:

• Zhao L, Liu P, Mao M, Zhang S, Bigenwald C, Dutertre CA, Lehmann CHK, Pan H, Paulhan N, Amon L, Buqué A, Yamazaki T, Galluzzi L, Kloeckner B, Silvin A, Pan Y, Chen H, Tian AL, Ly P, Dudziak D, Zitvogel L, Kepp O, Kroemer G. BCL2 Inhibition Reveals a Dendritic Cell-Specific Immune Checkpoint That Controls Tumor Immunosurveillance. Cancer Discov. 2023 Nov 1;13(11):2448-2469.

  Zhang S, Zhao L, Guo M, Liu P, Li S, Xie W, Tian AL, Pol JG, Chen H, Pan H, Mao M, Li Y, Zitvogel L, Jin Y, Kepp O, Kroemer G. Anticancer effects of ikarugamycin and astemizole identified in a screen for stimulators of cellular immune responses. J Immunother Cancer. 2023 Jul;11(7):e006785.

  Fidelle M, Rauber C, Alves Costa Silva C, Tian AL, Lahmar I, de La Varende AM, Zhao L, Thelemaque C, Lebhar I, Messaoudene M, Pizzato E, Birebent R, Mbogning Fonkou MD, Zoppi S, Reni A, Dalban C, Leduc M, Ferrere G, Durand S, Ly P, Silvin A, Mulder K, Dutertre CA, Ginhoux F, Yonekura S, Roberti MP, Tidjani- Alou M, Terrisse S, Chen J, Kepp O, Schippers A, Wagner N, Suárez-Gosálvez J, Kobold S, Fahrner JE, Richard C, Bosq J, Lordello L, Vitali G, Galleron N, Quinquis B, Le Chatelier E, Blanchard L, Girard JP, Jarry A, Gervois N, Godefroy E, Labarrière N, Koschny R, Daillère R, Besse B, Truntzer C, Ghiringhelli F, Coatnoan N, Mhanna V, Klatzmann D, Drubay D, Albiges L, Thomas AM, Segata N, Danlos FX, Marabelle A, Routy B, Derosa L, Kroemer G, Zitvogel L. A microbiota- modulated checkpoint directs immunosuppressive intestinal T cells into cancers. Science. 2023 Jun 9;380(6649):eabo2296.

  Yonekura S, Terrisse S, Alves Costa Silva C, Lafarge A, Iebba V, Ferrere G, Goubet AG, Fahrner JE, Lahmar I, Ueda K, Mansouri G, Pizzato E, Ly P, Mazzenga M, Thelemaque C, Fidelle M, Jaulin F, Cartry J, Deloger M, Aglave M, Droin N, Opolon P, Puget A, Mann F, Neunlist M, Bessard A, Aymeric L, Matysiak-Budnik T, Bosq J, Hofman P, Duong CPM, Ugolini S, Quiniou V, Berrard S, Ryffel B, Kepp O, Kroemer G, Routy B, Lordello L, Bani MA, Segata N, Yengej FY, Clevers H, Scoazec JY, Pasolli E, Derosa L, Zitvogel L. Cancer Induces a Stress Ileopathy Depending on β-Adrenergic Receptors and Promoting Dysbiosis that Contributes to Carcinogenesis. Cancer Discov. 2022 Apr 1;12(4):1128-1151. 

  Pihán P, Lisbona F, Borgonovo J, Edwards-Jorquera S, Nunes-Hasler P, Castillo K, Kepp O, Urra H, Saarnio S, Vihinen H, Carreras-Sureda A, Forveille S, Sauvat A, De Giorgis D, Pupo A, Rodríguez DA, Quarato G, Sagredo A, Lourido F, Letai A, Latorre R, Kroemer G, Demaurex N, Jokitalo E, Concha ML, Glavic Á, Green DR, Hetz C. Control of lysosomal-mediated cell death by the pH-dependent calcium channel RECS1. Sci Adv. 2021 Nov 12;7(46):eabe5469. 

  Zhao L, Liu P, Xie W, Zhang S, Thieme S, Zitvogel L, Kroemer G, Kepp O. A genotype-phenotype screening system using conditionally immortalized immature dendritic cells. STAR Protoc. 2021 Aug 12;2(3):100732.

  Wu Q, Tian AL, Li B, Leduc M, Forveille S, Hamley P, Galloway W, Xie W, Liu P, Zhao L, Zhang S, Hui P, Madeo F, Tu Y, Kepp O, Kroemer G. IGF1 receptor inhibition amplifies the effects of cancer drugs by autophagy and immune-dependent mechanisms. J Immunother Cancer. 2021 Jun;9(6):e002722. 

  Kepp O, Bezu L, Yamazaki T, Di Virgilio F, Smyth MJ, Kroemer G, Galluzzi L. ATP and cancer immunosurveillance. EMBO J. 2021 Jul 1;40(13):e108130.